Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 1. Expression levels of S100B in normal and tumor tissue. The expression of S100B was assessed from the Oncomine database. (A) TCGA breast dataset; the bars are labeled as follows: 1, breast (normal, n=61); 2, invasive ductal breast carcinoma (n=389); 3, invasive breast carcinoma (n=76); mixed lobular and ductal breast carcinoma (n=7); 5, invasive lobular breast carcinoma (n=36). (B) The Ma et al breast dataset (19); the bars are labeled as follows: 1, breast carcinoma (n=14); 2, ductal breast carcinoma in situ (n=9); 3, invasive ductal breast carcinoma (n=9). Boxplot shows the z-score of S100B expression according to the raw data from Oncomine. Error bars represent standard deviation (SD). The statistically significant differences between each group were examined with one-way ANOVA (***P<0.001). (C) S100B expression in different types of breast cancer examined via the GOBO database. (D) S100B expres- sion was examined in estrogen receptor (ER)-negative (ER-neg) and ER-positive (ER-pos) breast cancer using the GOBO database. The number above each bar indicates the sample size in each group. (E) mRNA expression of S100B in breast cancer cell lines. The error bars represent SD.

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Labeling, In Situ, Standard Deviation

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 2. S100B treatment inhibits cell migration. Transwell migration assay in (A) MDA-MB-231 and (B) Hs578T and (C) MCF-7 cells. Images and quanti- fication results are shown. Images of wound healing assay in (D) MDA-MB-231 and (E) MCF-7 cells. The quantification of wound healing assay in both cells is shown in the lower panel in the images. Error bars represent SD (one-way ANOVA; **p<0.01 and ***p<0.001).

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Migration, Transwell Migration Assay, Wound Healing Assay

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 3. S100B treatment induces mesenchymal-epithelial transition (MET) in MDA-MB-231 cells. (A and B) Western blot analysis was used to examine the protein expression levels of MET signaling pathway-related molecules. Band intensity was normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The relative value of the control (0 nM) was set to 1. Error bars represent SD (t-test; *p<0.05 and **p<0.01).

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Western Blot, Expressing, Control

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 4. Evaluation of the association between S100B expression and survival curve. (A) S100B expression in the low-risk (green) and high-risk (red) group. The box plot is adapted from the SurvExpress database and shows S100B expression in patients. (B) Kaplan-Meier distant recurrence curve was created using the SurvExpress database to analyze the sample from a GEO dataset (GSE9893). The low-risk (n=67) and high-risk (n=67) groups are shown in green and red, respectively. (C) Analyzing the overall survival rate in all breast cancer patients, (D) estrogen receptor (ER)-positive breast cancer patients, and (E) ER-negative breast cancer patients in ‘breast invasive carcinoma TCGA’ from the SurvExpress database. The green and red lines indicate a high and low S100B expression, respectively. Evaluation of the distant metastasis-free survival (DMFS) curve comparing the patients with a high (red) and low (black) S100B expression in (F) all breast cancer patients, (G) ER-positive breast cancer patients, (H) ER-negative breast cancer patients and (I) endocrine therapy-treated breast cancer patients using the KM plotter database. The hazard ratio (HR) and log-rank P-values are shown in each panel.

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 5. Evaluation of whether p53 expression in human breast cancer is associated with S100B expression and patient survival. (A) Expression of S100B in patients with or without p53 mutation. Raw data were adapted from the TCGA breast dataset. Distant metastasis-free survival (DMFS) analysis in breast cancer patients with (B) p53 mutation and (C) p53 wild-type. The hazard ratio (HR) and log-rank P-value are shown in each panel.

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Mutagenesis

Journal: International journal of oncology

Article Title: S100B expression in breast cancer as a predictive marker for cancer metastasis.

doi: 10.3892/ijo.2017.4226

Figure Lengend Snippet: Figure 6. Summary of the S100B-mediated effects in breast cancer. Patients with breast cancer expressing high levels of S100B exhibited a good prognosis and a low metastatic rate. When the breast cancer cells are treated with S100B, the migration ability was inhibited and the epithelial phenotype was induced.

Article Snippet: After scratching, cell debris was removed by washing twice with phosphate-buffered saline (PBS) and the cells were then incubated in serum-free L-15 medium containing 0, 0.1 and 1 nM recombinant human S100B protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Migration